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nunc slide flasks  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nunc slide flasks
    Nunc Slide Flasks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/slide+flasks/nunc+slide+flasks/10__1016_slash_j__cirep__2025__200205-112-10-13
    Average 90 stars, based on 1 article reviews
    nunc slide flasks - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Differential Radiomodulating Action of Olea europaea L. cv. Caiazzana Leaf Extract on Human Normal and Cancer Cells: A Joint Chemical and Radiobiological Approach
    Article Snippet: .. To assess the radiomodulating properties of OLC, the water-soluble extract was added at a final concentration of 12.5 μL/mL to exponentially growing cells seeded at appropriate densities in T12.5 or T25 tissue culture flasks for senescence time-course experiments (around 1.5 × 10 4 and 5 × 10 4 cells/flask, respectively) or in NuncTM (Thermo Fisher Scientific, Waltham, MA, USA) slide flasks (around 2 × 10 4 cells/slide flask) for DNA damage evaluation by the CBMN assay. ..

    Immunofluorescence:

    Article Title: Hypoxia Modulates Radiosensitivity and Response to Different Radiation Qualities in A549 Non-Small Cell Lung Cancer (NSCLC) Cells.
    Article Snippet: Geyer GmbH, Renningen, Germany) at a density of 5000 cells/cm2, using Alpha-Minimally Essential Medium (α-MEM; PAN Biotech, Aidenbach, Germany) containing 10% (v/v) dialyzed Fetal Bovine Serum (FBS; PAN Biotech), 2% (v/v) sterile glucose solution (0.94 mol/L), 1% (v/v) Penicillin (10,000 U/mL)/Streptomycin (10 mg/mL) (PAN Biotech), 1% (v/v) Neomycin/Bacitracin (Biochrom AG, Berlin, Germany), and 1% (v/v) Amphotericin (250 μg/mL) (PAN Biotech). .. For immunofluorescence experiments (Section 4.4), cells were cultured on glass coverslips (∅10 mm, Paul Marienfeld GmbH & Co. KG, Lauda Königshofen, Germany) placed in Petri dishes (∅3 cm) (Greiner Bio-One GmbH, Frickenhausen, Germany) and in 9 cm2 slide flasks (ThermoFisher Scientific, Waltham, MA, USA) using the same cell density and culture medium as described above. .. The cells were incubated at 37 ◦C and saturated humidity either under normoxia (20% O2) in a CO2 incubator (5% CO2; Heraeus HERAcell 150, Thermo Fisher Scientific, Karlsruhe, Germany) or under hypoxia (1% O2) in an InvivO2 400 hypoxia workstation (Baker Ruskinn, South Wales, UK) flushed with 5% CO2, 1% O2, and 94% N2.

    Article Title: Hypoxia Modulates Radiosensitivity and Response to Different Radiation Qualities in A549 Non-Small Cell Lung Cancer (NSCLC) Cells
    Article Snippet: Geyer GmbH, Renningen, Germany) at a density of 5000 cells/cm 2 , using Alpha-Minimally Essential Medium (α-MEM; PAN Biotech, Aidenbach, Germany) containing 10% ( v / v ) dialyzed Fetal Bovine Serum (FBS; PAN Biotech), 2% ( v / v ) sterile glucose solution (0.94 mol/L), 1% ( v / v ) Penicillin (10,000 U/mL)/Streptomycin (10 mg/mL) (PAN Biotech), 1% ( v / v ) Neomycin/Bacitracin (Biochrom AG, Berlin, Germany), and 1% ( v / v ) Amphotericin (250 μg/mL) (PAN Biotech). .. For immunofluorescence experiments , cells were cultured on glass coverslips (∅10 mm, Paul Marienfeld GmbH & Co. KG, Lauda Königshofen, Germany) placed in Petri dishes (∅3 cm) (Greiner Bio-One GmbH, Frickenhausen, Germany) and in 9 cm 2 slide flasks (ThermoFisher Scientific, Waltham, MA, USA) using the same cell density and culture medium as described above. .. The cells were incubated at 37 °C and saturated humidity either under normoxia (20% O 2 ) in a CO 2 incubator (5% CO 2 ; Heraeus HERAcell 150, Thermo Fisher Scientific, Karlsruhe, Germany) or under hypoxia (1% O 2 ) in an InvivO 2 400 hypoxia workstation (Baker Ruskinn, South Wales, UK) flushed with 5% CO 2 , 1% O 2 , and 94% N 2 .

    Article Title: Unraveling astrocyte behavior in the space brain: Radiation response of primary astrocytes
    Article Snippet: .. For immunofluorescence staining, 1 × 10 4 astrocytes were seeded on sterilized glass coverslips (Ø 10 mm, Carl Roth GmbH & Co.KG, Karlsruhe, Germany) or slide flasks (ThermoFisher Scientific) and grown for 3 days. ..

    Cell Culture:

    Article Title: Hypoxia Modulates Radiosensitivity and Response to Different Radiation Qualities in A549 Non-Small Cell Lung Cancer (NSCLC) Cells.
    Article Snippet: Geyer GmbH, Renningen, Germany) at a density of 5000 cells/cm2, using Alpha-Minimally Essential Medium (α-MEM; PAN Biotech, Aidenbach, Germany) containing 10% (v/v) dialyzed Fetal Bovine Serum (FBS; PAN Biotech), 2% (v/v) sterile glucose solution (0.94 mol/L), 1% (v/v) Penicillin (10,000 U/mL)/Streptomycin (10 mg/mL) (PAN Biotech), 1% (v/v) Neomycin/Bacitracin (Biochrom AG, Berlin, Germany), and 1% (v/v) Amphotericin (250 μg/mL) (PAN Biotech). .. For immunofluorescence experiments (Section 4.4), cells were cultured on glass coverslips (∅10 mm, Paul Marienfeld GmbH & Co. KG, Lauda Königshofen, Germany) placed in Petri dishes (∅3 cm) (Greiner Bio-One GmbH, Frickenhausen, Germany) and in 9 cm2 slide flasks (ThermoFisher Scientific, Waltham, MA, USA) using the same cell density and culture medium as described above. .. The cells were incubated at 37 ◦C and saturated humidity either under normoxia (20% O2) in a CO2 incubator (5% CO2; Heraeus HERAcell 150, Thermo Fisher Scientific, Karlsruhe, Germany) or under hypoxia (1% O2) in an InvivO2 400 hypoxia workstation (Baker Ruskinn, South Wales, UK) flushed with 5% CO2, 1% O2, and 94% N2.

    Article Title: Hypoxia Modulates Radiosensitivity and Response to Different Radiation Qualities in A549 Non-Small Cell Lung Cancer (NSCLC) Cells
    Article Snippet: Geyer GmbH, Renningen, Germany) at a density of 5000 cells/cm 2 , using Alpha-Minimally Essential Medium (α-MEM; PAN Biotech, Aidenbach, Germany) containing 10% ( v / v ) dialyzed Fetal Bovine Serum (FBS; PAN Biotech), 2% ( v / v ) sterile glucose solution (0.94 mol/L), 1% ( v / v ) Penicillin (10,000 U/mL)/Streptomycin (10 mg/mL) (PAN Biotech), 1% ( v / v ) Neomycin/Bacitracin (Biochrom AG, Berlin, Germany), and 1% ( v / v ) Amphotericin (250 μg/mL) (PAN Biotech). .. For immunofluorescence experiments , cells were cultured on glass coverslips (∅10 mm, Paul Marienfeld GmbH & Co. KG, Lauda Königshofen, Germany) placed in Petri dishes (∅3 cm) (Greiner Bio-One GmbH, Frickenhausen, Germany) and in 9 cm 2 slide flasks (ThermoFisher Scientific, Waltham, MA, USA) using the same cell density and culture medium as described above. .. The cells were incubated at 37 °C and saturated humidity either under normoxia (20% O 2 ) in a CO 2 incubator (5% CO 2 ; Heraeus HERAcell 150, Thermo Fisher Scientific, Karlsruhe, Germany) or under hypoxia (1% O 2 ) in an InvivO 2 400 hypoxia workstation (Baker Ruskinn, South Wales, UK) flushed with 5% CO 2 , 1% O 2 , and 94% N 2 .

    Article Title: Long-Term Simulation of Microgravity Induces Changes in Gene Expression in Breast Cancer Cells
    Article Snippet: The cells were grown in RPMI 1640 medium (Thermo Fisher Scientific, Nærum, Denmark), supplemented with 10% fetal calf serum (FCS) (Sigma-Aldrich Chemie GmbH, Munich, Germany) and 1% penicillin/streptomycin (Thermo Fisher Scientific), and maintained under standard cell culture conditions at 37 °C and 5% CO 2 . .. One day prior to the RPM experiment, 1 × 10 6 cells were counted and seeded into T25 cm 2 vented cell culture flasks (Sarstedt, Nümbrecht, Germany) and 2.5 × 10 5 cells were seeded into the slide flasks (Thermo Fisher Scientific) for F-actin staining and immunohistochemical investigations. ..

    Staining:

    Article Title: Unraveling astrocyte behavior in the space brain: Radiation response of primary astrocytes
    Article Snippet: .. For immunofluorescence staining, 1 × 10 4 astrocytes were seeded on sterilized glass coverslips (Ø 10 mm, Carl Roth GmbH & Co.KG, Karlsruhe, Germany) or slide flasks (ThermoFisher Scientific) and grown for 3 days. ..

    Article Title: Long-Term Simulation of Microgravity Induces Changes in Gene Expression in Breast Cancer Cells
    Article Snippet: The cells were grown in RPMI 1640 medium (Thermo Fisher Scientific, Nærum, Denmark), supplemented with 10% fetal calf serum (FCS) (Sigma-Aldrich Chemie GmbH, Munich, Germany) and 1% penicillin/streptomycin (Thermo Fisher Scientific), and maintained under standard cell culture conditions at 37 °C and 5% CO 2 . .. One day prior to the RPM experiment, 1 × 10 6 cells were counted and seeded into T25 cm 2 vented cell culture flasks (Sarstedt, Nümbrecht, Germany) and 2.5 × 10 5 cells were seeded into the slide flasks (Thermo Fisher Scientific) for F-actin staining and immunohistochemical investigations. ..

    Immunohistochemical staining:

    Article Title: Long-Term Simulation of Microgravity Induces Changes in Gene Expression in Breast Cancer Cells
    Article Snippet: The cells were grown in RPMI 1640 medium (Thermo Fisher Scientific, Nærum, Denmark), supplemented with 10% fetal calf serum (FCS) (Sigma-Aldrich Chemie GmbH, Munich, Germany) and 1% penicillin/streptomycin (Thermo Fisher Scientific), and maintained under standard cell culture conditions at 37 °C and 5% CO 2 . .. One day prior to the RPM experiment, 1 × 10 6 cells were counted and seeded into T25 cm 2 vented cell culture flasks (Sarstedt, Nümbrecht, Germany) and 2.5 × 10 5 cells were seeded into the slide flasks (Thermo Fisher Scientific) for F-actin staining and immunohistochemical investigations. ..



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